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Figure 4 | Veterinary Research

Figure 4

From: Binding and cleavage of pro-urokinase by a tegument extract of Fasciola hepatica newly excysted juveniles activate the host fibrinolytic system

Figure 4

Stimulation of u-PA generation from pro-u-PA by FhNEJ-Teg proteins enhances plasmin generation from the host PLG. Pro-u-PA was incubated in the presence or absence of FhNEJ-Teg and PLG, and plasmin generation was assessed by measuring the cleavage of a plasmin-specific chromogenic substrate (absorbance at 405 nm) over time. Wells containing u-PA and the plasmin-specific chromogen were used to control for unspecific activity of u-PA towards this substrate, and wells containing plasmin alone were used to control for substrate specificity. The figure shows a representative result of two independent experiments, where the bars indicate the means of three technical replicates ± SD. Asterisks indicate significant differences between pro-u-PA + PLG with and without FhNEJ-Teg (***p ≤ 0.001; one-way ANOVA). Note that the data points corresponding to FhNEJ-Teg + pro-u-PA, FhNEJ-Teg + PLG, FhNEJ-Teg, pro-u-PA, PLG, u-PA and Plm chromosomes only overlap.

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Veterinary Research

ISSN: 1297-9716