Figure 1

25HC induces LDs accumulation and inhibits cholesterol abundance. A LLC-PK1 cells were infected with PDCoV (MOI = 0.1) and treated with 50 μM 25HC for 12 h. Lipid droplets (LD) were stained green with BODIPY (493/503), and nuclei were stained blue with DAPI. Scale bar, 10 μm. B Average number of LDs per cell was measured using Image J. C Free cholesterol in cells was stained blue with Filipin III. Scale bar, 10 μm. D The mean fluorescence intensity (MFI) of cholesterol in panel C was analyzed using Image J. E, F The cellular concentrations of Triglyceride (TG) and Free cholesterol (FC) were measured in LLC-PK1 cells infected with PDCoV and treated with 25HC for 12 h. Error bars, mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.