Figure 2

PDCoV induces active lipophagy in LLC-PK1 cells. A, B LLC-PK1 cells were infected with PDCoV (MOI = 0.1) and treated with 200 μM Oleic acid (OA) for 12 h. A LC3, p62 and PLIN2 expression were analyzed by western blot, Tubulin was used as a loading control. B The immunoblot bands in panel A were quantified by Image J. C–E LLC-PK1 cells were infected with PDCoV (MOI = 0.1) and treated with 200 μM Oleic acid (OA), 200 nM Bafilomycin A1 (Baf A1) for 12 h. C The indicated protein expression were determined by western blot analysis, GAPDH and Tubulin were used as loading control. D The immunoblot bands in panel C were quantified by Image J. E The relative mRNA expression of PDCoV N, ISG15 and MX1 were measured using qPCR analysis. Error bars, mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ns represents not significant.