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Figure 6. | Veterinary Research

Figure 6.

From: The cholesterol metabolite 25-hydroxycholesterol suppresses porcine deltacoronavirus via lipophagy inhibition and mTORC1 modulation

Figure 6.

25HC negatively regulates TFEB expression mediated by mTOR. A, B LLC-PK1 cells were infected with PDCoV (MOI = 0.1) and treated with 50 μM 25HC for indicated time. A Indicated protein expressions were measured using western blot, with Tubulin being a loading control. B Immunoblot bands in panel A were quantified by Image J. C–E LLC-PK1 cells were infected with PDCoV (MOI = 0.1) and treated with 50 μM 25HC, 0.5 μM Torin1 for 12 h. C Indicated protein expressions were measured using western blot, GAPDH was used as a loading control. D Immunoblot bands in panel C were quantified by Image J. E Relative mRNA expression of PDCoV N was analyzed by qPCR. Error bars, mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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Veterinary Research

ISSN: 1297-9716