Figure 7.

25HC inhibits PDCoV infection in a piglet model. 25HC (10 mg/kg) or vehicle (HβCD) was administered after PDCoV infection in piglets. A The diarrhea score, body temperature and body weight of piglets. B The number of leukocytes in blood. C The anatomical observation of piglets after they were sacrificed. The red arrows indicated the hyperemia in the intestines. D The H&E staining and histopathological score of Jejunum and ileum in piglets. Scale bar, 20 μm. E The PDCoV N protein was stained red using specific antibody and nuclei were stained blue with DAPI. Scale bar, 20 μm. The MFI of PDCoV N were analyzed by image J. F Relative mRNA expression of PDCoV N in jejunum and ileum were analyzed by qPCR. G The concentration of Free cholesterol (FC), Total cholesterol (TC) and triglyceride (TG) in serum were analyzed by commercial kits. H The porcine IgA and IFN-β content were measured by ELISA. I The ultrastructure of jejunum and ileum were observed using transmission electron microscopy. The scale bars were 2 μm and 0.5 μm, respectively. The area of lysosome was quantified using Fiji-image J. J The indicated protein expressions in jejunum and ileum were measured using western blot, GAPDH was used as a loading control. K The indicated gene expressions in jejunum and ileum were detected using qPCR. Error bars, mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.