Table 1 Antibodies used for western blot
Antibody | Product code | Manufacturer | Dilution |
|---|---|---|---|
LC3 | 14600-1-AP | Proteintech | 1:2000 |
Beclin1 | 11306-1-AP | Proteintech | 1:1000 |
ATG5 | 10181-2-AP | Proteintech | 1:1000 |
p62 | 18420-1-AP | Proteintech | 1:1000 |
LIPA | 12956-1-AP | Proteintech | 1:1000 |
PLIN2 | 15294-1-AP | Proteintech | 1:1000 |
LAMP1 | 21997-1-AP | Proteintech | 1:10Â 000 |
Cathepsin D | 55021-1-AP | Proteintech | 1:3000 |
ATGL | 55190-1-AP | Proteintech | 1:500 |
TFEB | 13372-1-AP | Proteintech | 1:2000 |
NPC1 | 13926-1-AP | Proteintech | 1:500 |
SREBP2 | 28212-1-AP | Proteintech | 1:2000 |
P70S6K | 14485-1-AP | Proteintech | 1:2000 |
p-P70S6K | 28735-1-AP | Proteintech | 1:2000 |
GAPDH | 60004-1-Ig | Proteintech | 1:5000 |
β-actin | 60008-1-AP | Proteintech | 1:5000 |
Tubulin | 10068-1-AP | Proteintech | 1:5000 |
Histone-H3 | 17168-1-AP | Proteintech | 1:10Â 000 |
PDCoV-N | SD-4-5 | Medgene Labs | 1:500 |
4EBP1 | #9452 | Cell Signaling Technology | 1:1000 |
p-4EBP1 | #2855 | Cell Signaling Technology | 1:1000 |
mTOR | T55306 | Abmart | 1:1000 |
Phosphorylation-mTOR | ab109268 | ABCAM | 1:1000 |