The cholesterol metabolite 25-hydroxycholesterol suppresses porcine deltacoronavirus via lipophagy inhibition and mTORC1 modulation

Table 2 Primers used for qPCR

Gene	Direction	Sequence (5′ → 3′)	Production Size
PDCoV N	Forward	AACTTTCAGGCAGGGGCAAT	134 bp
PDCoV N	Reverse	GGTTTGGTGGGTGGCTCATA	134 bp
GAPDH	Forward	ACATGGCCTCCAAGGAGTAAGA	106 bp
GAPDH	Reverse	GATCGAGTTGGGGCTGTGACT	106 bp
IFN-β	Forward	TTCGAGGTCCCTGAGGAGATT	176 bp
IFN-β	Reverse	TCCATCTGCCCATCAAGTTCC	176 bp
TFEB	Forward	GGACACCTGAACTTGGGACAA	83 bp
TFEB	Reverse	TGCTGGCTCCCTATCTCTGA	83 bp
NPC1	Forward	GCTACCGTGTGTTCCCCTAC	92 bp
NPC1	Reverse	GGACACGCCGAGGTTAAAGA	92 bp
MX1	Forward	GTGGAGAAAAGTCACAAAACAGGGC	288 bp
MX1	Reverse	TTTGCCCTTCCATTCGTCTTCT	288 bp
ISG15	Forward	GGTGAGGAACGACAAGGGTC	177 bp
ISG15	Reverse	GGCTTGAGGTCATACTCCCC	177 bp
ISG20	Forward	CAGGACAGTGTGTTGGGACA	101 bp
ISG20	Reverse	ACAGGCAGCCCTCATTGTTC	101 bp

ISSN: 1297-9716