Table 1 Primers used for plasmid construction
Gene name | Forward primer sequence (5ʹ–3ʹ) | Reverse primer sequence (5ʹ–3ʹ) | |
|---|---|---|---|
HY12-2C HY12-VP1 HY12-VP2 HY12-VP3 HY12-VP4 HY12-2A HY12-2B HY12-3AB HY12-3C HY12-3D 2C (aa 1–125) 2C (aa 126–253) | CCGGAATTCTCAGACAATTGGAT CCGGAATTCAATGACCCAGGGAA CCGGAATTCTCTCCGTCAGCAGAA CCGGAATTCGGCCTTCCAACAAA CCGGAATTCATGGGCGGTCAGTT CCGGAATTCGGGCCTTTTGGACA CCGGAATTCGGCATTACTGATTAT CCGGAATTCGGCCCCGTAACCTA CCCTCGAGGGACCACTCTTTGA CCGGAATTCGGGCAAATAGAATAC GCGTCGACTCAGACAATTGGATAA GCGTCGACCTCATACATGGCTCTCC | ATTTGCGGCCGCTTATTGAAAAAGG ATTTGCGGCCGCGTACGAGGTGAG ATTTGCGGCCGCTTGGTTTGATGCAA ATTTGCGGCCGCCTGTAGTGCGGCTG ATTTGCGGCCGCTTTCAGCGGAACG ATTTGCGGCCGCTTGCTCCATAGCGT ATTTGCGGCCGCTTGCCTCTCTGCCA ATTTGCGGCCGCTTGAGTTTGAACCT ATTTGCGGCCGCTTGTTCAACAGTAA ATTTGCGGCCGCTTAGAAAGAATCT CGGGATCCTTATGCACATACGGGTTC CGGGATCCTTATGTCTTTCTGTACT | |
2C (aa 254–329) 2C (aa 1–121) 2C (aa 1–117) 2C (aa 1–113) p65 p65 (aa 1–290) p65 (aa 291–551) p65 (aa 1–194) p65 (aa 195–290) IKBKB | GCGTCGACTATAAGAAGAATGGAT GCGTCGACTCAGACAATTGGATAA GCGTCGACTCAGACAATTGGATAA GCGTCGACTCAGACAATTGGATAA CGCGGATCCATGGACGAACTGTTCC CGCGGATCCATGGACGAACTGTTCC CGGGATCCATGGATACAGACGATC CGCGGATCCATGGACGAACTGTTCC CGCGGATCCATAAGATCTGCCGAG CCAAGCTTATGAGAAGGCTGACCC | CGGGATCCTTATTGAAAAAGAGCCTC CGGGATCCTTATTCAATGCGATTC CGGGATCCTTACTTGGTCTTGAACTG CGGGATCCTTACTGCATGGCACCGAG TGCTCTAGAGGAGCTGATCTGACT TGCTCTAGATGGCAGGTACTGGAATT TGCTCTAGAGCTGATCTGACTCAGC TGCTCTAGAGAGCTCGGCAGTGTT TGCTCTAGATGGCAGGTACTGGAATT GCTCTAGATGAGGCCTGCTCCAGG |