Figure 2

The phages lysin ClyL and Lys0859 could effectively lyse bacteria isolated from sows with endometritis and bovine mastitis. A–D The antibacterial activity of Lys0859 (50 μg/mL) and ClyL (50 μg/mL) against clinical bacteria isolated from sows subjected to endometritis at 37 °C for 1 h. The values of turbidity decrease were classified into three parts: a decrease in turbidity > 50%, a 20% ≤ decrease in turbidity ≤ 50%, and a decrease in turbidity < 20%. The proportions of the three parts are presented in a circular figure. The proportions of the decrease in ClyL turbidity against Staphylococcus and Streptococcus are listed in A and C, respectively. The proportions of Lys0859 turbidity decreases against Staphylococcus and Streptococcus are listed in B and D, respectively. E The antibacterial activity of Lys0859 (50 μg/mL) and ClyL (50 μg/mL) against clinical bacteria isolated from bovine mastitis. F, G The bacterial count test was used to evaluate the bactericidal activity of Lys0859 (50 μg/mL) and ClyL (50 μg/mL) against Staphylococcus and Streptococcus strains isolated from sows with endometritis (F) and bovine mastitis (G). Significant differences between the Lys0859 groups and ClyL groups were determined by Student’s t test (** P < 0.01, and *** P < 0.001). The data represent the mean ± SD of three independent experiments.