Figure 2

Methylation levels of the MyD88 promoter in mice challenged with T. spiralis and DNMTs and TETs activities assays. A MethPrimer software prediction of CGI of the MyD88 promoter region. The blue areas on the picture indicate the potential CGI. B The distribution of CpG dinucleotides in the CGI of MyD88. The CGI has a cluster of 23 individual CpG sites, and each yellow rectangle represents a CpG site. C The PCR products of MyD88 promoter with methylation primers. D: The methylation status of the promoter of MyD88 on 0 and 5 dpi, respectively. Each row corresponded to a single template DNA molecule cloned. Eight colonies from each ligation were randomly picked and sequenced. Each circle represented a CpG site (black circles represented methylated CpG site; white circles were unmethylated CpG site; yellow circles were mutant CpG site). E The methylation level of the MyD88 promoter on 0 and 5 dpi, respectively. F The mutant pattern of the CpG site with G/A mutation. The mutation sites measured by sequencing are framed with red. G The mutant level of the MyD88 promoter on 0 and 5 dpi, respectively. H, I Bar charts showing DNMTs (H) and TETs (I) activities of spleen tissues in 0 and 5 dpi, respectively. *P < 0.05, **P < 0.01, ***P < 0.001.