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Figure 6 | Veterinary Research

Figure 6

From: MyD88 inhibitor TJ-M2010-5 alleviates spleen impairment and inflammation by inhibiting the PI3K/miR-136-5p/AKT3 pathway in the early infection of Trichinella spiralis

Figure 6

TJ-M2010-5 reduced inflammation on RAW264.7 cells stimulated with LPS. A CCK8 assay. *P < 0.05, **P < 0.01, ***P < 0.001. B Schematic diagram of RAW264.7 inflammatory cell model induced by LPS. C IL-6, IL-1β and TNF-α mRNA expression of different groups. D miR-136-5p mRNA expression of different groups. E TJ-M2010-5 effects on the NO production in RAW264.7 cells stimulated by LPS. F, G Representative plots of M1 and M2 macrophages pre-treatment with 10 and 20 μM TJ-M2010-5. The M1/M2 ratio was determined by flow cytometry. H–I Western blotting analysis of iNOS, pPI3K, PI3K, pAKT, AKT, MyD88 and AKT3 protein expression. The protein intensity was analysed by using ImageJ. Compared with control group: *P < 0.05, **P < 0.01, ***P < 0.001; Compared with LPS group: #P < 0.05, ##P < 0.01, ###P < 0.001.

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Veterinary Research

ISSN: 1297-9716