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Figure 2 | Veterinary Research

Figure 2

From: Usutu virus continues to spread across Europe: first report of multiple molecular detections of the USUV Africa 2 and Africa 3 lineages in free-living and captive birds in Poland, July–November 2023

Figure 2

Phylogenetic analysis of the USUV-positive samples from Poland, July–November 2023. Maximum-likelihood tree based on the partial nucleotide sequence of the NS5 gene (471 bp), demonstrating the belonging of the Polish virus strains to the USUV Africa 2 (pink) and Africa 3 (blue) genetic lineages. IQ-TREE software [11] was used to generate the phylogenetic tree (best-fit model: K2P + I, 1000 replicates) after including nucleotide sequences with 99% similarity to the Polish viruses [10] and other lineage-specific sequences [1, 3]. Two USUV-positive samples were excluded from the phylogenetic analysis because of their shorter lengths at the 3ʹ and 5ʹ ends (PQ039622, PQ039642). Bootstrap values above 70 are shown.

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Veterinary Research

ISSN: 1297-9716