Table 3 Plasmids construction in this study
Plasmids | Genotype/Description | Source |
|---|---|---|
pRS416-GAL1-ScRce1 | Gal1 promoter, 3 × flag-ScRce1, URA3, ampicillin | This study |
pRS416-GAL1-LI0758 | Gal1 promoter, 3 × flag-LI0758, URA3, ampicillin | This study |
pYES2/NTA-RipI | Gal1 promoter, EGFP-RipI, URA3, ampicillin | Lu Lab |
pGBKT7 | T7 promoter, c-Myc, kanamycin | Clontech |
pESC-Leu | Gal1 promoter, c-Myc, ampicillin | Agilent |
pESC-Leu-EGFP-Ras2 | Gal1 promoter, c-Myc-EGFP-Ras2, ampicillin | This study |
pBAD24-mCherry | Arabinose Bad promoter, mCherry, ampicillin | Feng shao lab |
pRS416-mCherry-ScRce1 | Arabinose Bad promoter, mCherry-ScRce1, URA3, ampicillin | This study |
pRS416-mCherry-LI0758 | Arabinose Bad promoter, mCherry-LI0758, URA3, ampicillin | This study |
pCDNA3.1( +)-mCherry-KDEL | CMV promoter, signal- mCherry-KDEL, ampicillin | This study |
pEGFP-C1 | CMV promoter, EGFP, kanamycin | Clontech |
pEGFP-C1-LI0758 | CMV promoter, EGFP-LI0758, kanamycin | This study |
pEGFP-N1-LI0758 | CMV promoter, LI0758-EGFP, kanamycin | This study |