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Table 3 Plasmids construction in this study

From: Lawsonia intracellularis T3SS effector LI0758, an Rce1 ortholog, activates MAPK and NF-κB signaling in mammalian cells

Plasmids

Genotype/Description

Source

pRS416-GAL1-ScRce1

Gal1 promoter, 3 × flag-ScRce1, URA3, ampicillin

This study

pRS416-GAL1-LI0758

Gal1 promoter, 3 × flag-LI0758, URA3, ampicillin

This study

pYES2/NTA-RipI

Gal1 promoter, EGFP-RipI, URA3, ampicillin

Lu Lab

pGBKT7

T7 promoter, c-Myc, kanamycin

Clontech

pESC-Leu

Gal1 promoter, c-Myc, ampicillin

Agilent

pESC-Leu-EGFP-Ras2

Gal1 promoter, c-Myc-EGFP-Ras2, ampicillin

This study

pBAD24-mCherry

Arabinose Bad promoter, mCherry, ampicillin

Feng shao lab

pRS416-mCherry-ScRce1

Arabinose Bad promoter, mCherry-ScRce1, URA3, ampicillin

This study

pRS416-mCherry-LI0758

Arabinose Bad promoter, mCherry-LI0758, URA3, ampicillin

This study

pCDNA3.1( +)-mCherry-KDEL

CMV promoter, signal- mCherry-KDEL, ampicillin

This study

pEGFP-C1

CMV promoter, EGFP, kanamycin

Clontech

pEGFP-C1-LI0758

CMV promoter, EGFP-LI0758, kanamycin

This study

pEGFP-N1-LI0758

CMV promoter, LI0758-EGFP, kanamycin

This study