Figure 3

HnRNPC increases SVCV-P stability by suppressing K48-linked polyubiquitination. A–C HEK293 cells were transfected with the indicated plasmids. At 24 h post-transfection, the cells were harvested for Co-IP. D EPC cells were co-transfected with combinations of the indicated plasmids, including Myc-SVCV-P with GFP-hnRNPC (0.5, 1 or 2 µg). At 24 h post-transfection, the cells were harvested for immunoblotting. E, F HEK293 cells were co-transfected with GFP-SVCV-P, GFP-SVCV-P/HA-ub, or GFP-SVCV-P/HA-ub/Flag-hnRNPC (E) and HEK293 cells were co-transfected with GFP-SVCV-P/HA-ub-K48O, GFP-SVCV-P/HA-ub-K48O/Flag-hnRNPC, GFP-SVCV-P/HA-ub-K63O, or GFP-SVCV-P/HA-ub-K63O/Flag-hnRNPC (F). The cells were collected at 24 h post-transfection and used for the co-IP assay.