Figure 4

HnRNPC inhibits ifnα1 promoter activity induced by SVCV and poly(I:C). A–D EPC cells were co-transfected with pRL-TK (25 ng)/empty plasmid (250 ng) or Flag-hnRNPC (250 ng)/ifnφ1pro (250 ng) or ISRE-Luc (250 ng). At 24 h post-transfection, the cells were left untreated (MEM) or treated with SVCV or poly(I:C) (5 mg/mL). After 24 h, the cells were collected for the detection of luciferase activity. E–G EPC cells were transfected with vector or hnRNPC and then transfected with poly(I:C). After 24 h, qPCR was used to analyse the expression of ifn1, mx and isg15. β-actin was used as a control. The results are shown as the mean ± SD. Asterisks indicate statistically significant differences (*p < 0.05; **p < 0.01; N = 3).