Figure 6

The interaction between hnRNPC and RLR factors triggers the degradation of MITA. A HEK293 cells were co-transfected with GFP-hnRNPC plus Flag-RIG-I, Flag-MAVS, Flag-MITA, Flag-TBK1, Flag-IRF3, or Flag-IRF7. The cells were collected 24 h post-transfection and used for the co-IP assay. B EPC cells were co-transfected with Flag-RIG-I, Flag-MAVS, Flag-MITA, Flag-TBK1, Flag-IRF3, or Flag-IRF7 plus pEGFP-N1 or GFP-hnRNPC. At 24 h post-transfection, the cells were harvested for immunoblotting. C EPC cells were co-transfected with Flag-MITA plus pEGFP-N1 or GFP-hnRNPC. After 24 h, the cells were harvested for immunoblotting. D DrFIN cells were co-transfected with DsRed-hnRNPC/pEGFP-N1 or DsRed-hnRNPC/GFP-MITA. After 24 h, the cells were subjected to fluorescence microscopy.