Table 1 Information on the primers used in the study.
From: HnRNPC triggers the degradation of MITA to suppress the interferon-mediated antiviral response
Application | Primer | Sequence (5′-3′) |
|---|---|---|
Plasmid construction | Flag-hnRNPC-FW | CCCAAGCTTATGGCCAGTAATGTCACCAAC |
| Â | Flag-hnRNPC-BW | CCCAAGCTTATGGCCAGTAATGTCACCAAC |
| Â | Flag-hnRNPCRRM-FW | CCGGAATTCATGGCCAGTAATGTCACCAAC |
| Â | Flag-hnRNPCRRM-BW | CGGGGTACCGAATTAATATCGAGTACCTGTC |
| Â | Flag-hnRNPCC-FW | CCGGAATTCATGCTGGCTGGCGAGCCCAA |
| Â | Flag-hnRNPCC-BW | CGGGGTACCGAAGCATGATCTCCGTTGGCAC |
qRT‒PCR siRNA | Flag-MITAN-FW Flag-MITAN-BW Flag-MITAC-FW Flag-MITAC-BW GFP-SVCV P-FW GFP-SVCV P-BW GFP-MITAC-K27R-FW GFP-MITAC-K27R-BW GFP-MITAC-K45R-FW GFP-MITAC-K45R-BW GFP-MITAC-K53R-FW GFP-MITAC-K53R-BW GFP-MITAC-K62R-FW GFP-MITAC-K62R-BW GFP-MITAC-K100R-FW GFP-MITAC-K100R-BW GFP-MITAC-K149R-FW GFP-MITAC-K149R-BW GFP-MITAC-K220R-FW GFP-MITAC-K220R-BW ZF-HnRNPC-FW ZF-HnRNPC-BW SVCV-N-FW SVCV-N-BW SVCV-G-FW SVCV-G-BW sihnRNPC-1 sense (5′-3′) sihnRNPC-1antisense (5′-3′) sihnRNPC-2 sense (5′-3′) sihnRNPC-2antisense (5′-3′) sihnRNPC-3 sense (5′-3′) sihnRNPC-3antisense (5′-3′) | CGGAAGCTTATGGAGCAGAAGCTGATCAG CCCCGGTACGACGTTCACCTCTAAAGCTG CGGAAGCTTATGAGAGAATACTCTAGAAGGGA CGCGGTACCGTTTTGTTTCATTGCGCTAG CGGAAGCTTATGGAGCAGAAGCTGATCAG CGGGGTACCAGTCTGTACTTCTGATACA AATGCCAGAGTCCCAAGCCGACCTGGAGAGGAGGAC GCGCTTGGGACTCTGGCATTGAGGGGCAGAAGGAT GAAAACCTTCCGGATCTGCGGCTGGACAGGGCAGG CGCAGATCCGGAAGGTTTTCATGGAAGACCAC ACAGGGCAGGAGTGCGGCGACGCAGCTACACTAA CGCCGCACTCCTGCCCTGTCCAGCTTCAGATC TACACTAACAGCGTCTACCGGATCACCCACAACAA CGGTAGACGCTGTTAGTGTAGCTGCGTTTCCG GTTTGGCGAGAGAGAACGGCGGCAGCAGGTCCTG CGCCGTTCTCTCTCGCCAAACCCTGCACTGCTCTCCT GAGCTCTTCCAGAACCTGCGGCAGCAGGATGG CGCAGGTTCTGGAAGAGCTCTCTGGAGAGGTAATG AACCCATCTAGCGCAATGCGACAAAACGTACCGC GCATTGCGCTAGATGGGTTAAAATAATCGGTGGTCT AGTACGCCAACGAGAGGAAC ATGGTCTTTGACCGGTGAGG TCTGCCAAATCACCATACTCA CTGTCTTGCGTTCAGTGCTC ATCATTCAAAGGATTGCATCAG CATATGGCTCTAAATGAACAGAA GAGGCCAUCUUUAGUAAGUTT ACUUACUAAAGAUGGCCUCTT GGAACUGACUCAGAUUAAATT UUUAAUCUGAGUCAGUUCCTT GACAGGUACUCGAUAUUAATT UUAAUAUCGAGUACCUGUCTT |