Figure 6

PCA does not inactivate ASFV directly. A Schematic diagram of the ASFV inactivation assay. B to D The inactivation effects of PCA on ASFV. PCA (10 μM) and rASFV-Gluc/EGFP (MOI = 1) were incubated at 37 °C, and after 2 h, the virus-PCA mixture was diluted 100-fold to infect PAMs. EGFP expression was observed at 36 hpi, with representative fluorescence images captured (B). The average number of EGFP-positive cells per field of view was measured using the ImageJ software (C). Scale bars = 400 μm. At 72 hpi, the supernatants were collected to assess Gluc activities (D). ns, not significant.