Figure 1

Upregulation of bCoronin-1A promoted the intracellular survival of M.tb. A Protein sequence alignment of hCoronin-1A, mCoronin-1A and bCoronin-1A. The blue area indicates WD1-WD7 in the WD repeat domain, the gray area is the unique sequence, the green is the disordered sequence, and the orange part is the coiled-coil domain. B, C The mRNA and protein levels of bCoronin-1A were measured in EBL cells infected with H37Ra and BCG for 0, 5, 7, 10, 12 and 24 h by qRT-PCR and western blot (MOI = 20). D Immunofluorescence analysis of subcellular localization of bCoronin-1A before and after infection of RAW264.7 cells overexpressing bCoronin-1A with H37Ra (MOI = 20). The reagents used included rabbit anti-FLAG polyclonal antibody for labeling bCoronin-1A (red), mouse anti-tubulin polyclonal antibody (green) for labeling tublin and DAPI for nuclear staining (blue). E Detection of cell activity in RAW264.7 cells overexpressing bCoronin-1A before and after infection with H37Ra (MOI = 10). F CFU detection of H37Ra and BCG from infected RAW264.7 cells overexpressing bCoronin-1A at 4, 24, 48 and 72 h. Data were analyzed by t-test and presented as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.