Figure 2

DP71L inhibits antiviral immune signaling and the transcription of antiviral genes. A and B DP71L-Flag or control vector transiently and stably expressing PK-15 A and 3D4/21 B cells were infected with ADV-GFP (MOI = 1.0) and harvested at the indicated time points. Immunoblotting was performed to observe the phosphorylated and intact forms of TBK1, STAT1, p65, IRF3, IκBα, and the expression of DP71L-Flag. The internal control, β-actin, was used to ensure equal protein amounts in the samples. C and D DP71L-Flag or control vector transiently and stably expressing PK-15 C and 3D4/21 D cells were infected with ADV-GFP at a 1 MOI and harvested at indicated time points. The total RNA was extracted from the cells, and qRT-PCR was performed to assess the transcription levels of antiviral genes. Protein sizes are expressed in kilodaltons (kDa). All the data are representative of at least two independent experiments, each with similar results and the values are expressed as means ± SD for two biological replicates. Student’s t-test: *, P < 0.05; **, P < 0.01; ns, not significant.