Table 1 List of primers used for real-time PCR
From: Inhibition of STING-mediated type I IFN signaling by African swine fever virus DP71L
Target gene | Forward primer | Reverse primer |
|---|---|---|
DP71L | ATGGGGAGGCGGCGCAAAAA | TTACTGCTGCTCCAGTAGCT |
CP204L | TCTTTTGTGCAAGCATATACAGCTT | TGCACATCCTCCTTTGAAACAT |
I73R | ATGGAGACTCAGAAGTTGA | GTTTTTCCGTATCCAAAGC |
B646L | CCCAGGGGATAAAATGACTG | CACTGGTTCCCTCCACCGATA |
IFN-β | AAATCGCTCTCTGATGTGT | TGCTCCTTTGTTGGTATCG |
IFN-γ | CCATTCAAAGGAGCATGGAT | ATCCATGCTCCTTTGAATGG |
IL-6 | CACCGGTCTTGTGGAGTTTC | GTGGTGGCTTTGTCTGGATT |
IL-1β | GGGACTTGAAGAGAGAAGTGG | CTTTCCCTTGATCCCTAAGGT |
TNFα | TCACAGGGCAATGATCCC | GGGATCATTGCCCTGTGA |
MCP-1 | CAGAAGAGTCACCAGCAGCA | TCCAGGTGGCTTATGGAGTC |
MX-1 | TAGGCAATCAGCCATACG | GTTGATGGTCTCCTGCTTAC |
ISG15 | AAATCGCTCTCCTGATGTGT | TGCTCCTTTGTTGGTATCG |
ISG54 | ATGTGCACAGCAATCATGAGT | TTCCTCAGCTAAAGATACTAG |
ISG56 | CTGACTCACAGCAACCATG | CTTTCAGGTGTTTCACATAGG |
β-Actin | CTCGATCATGAAGTGCGACG | GTGATCTCCTTCTGCATCCTGT |