Table 1 Strains used in this study
Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
Strains | ||
S. suis | ||
P1/7 | S. suis reference strain, serotype 2, ST1 | [15] |
P1/7∆metQ | P1/7 strain with SSU1577 replaced by specR | This study |
P1/7∆metQ/Q+ | P1/7∆metQ strain with spec replaced by metQ gene and containing camR | This study |
P1/7Δgfp + | P1/7 expressing GFP fused to HlpA with tetR | Laboratory collection |
P1/7Δgfp + ∆metQ | P1/7Δgfp + with SSU1577 replaced by specR | This study |
E. coli | ||
BL21 (DE3) | Overexpression strain | Laboratory collection |
BL21-pET_MetQ | BL21 (DE3) strain with pET16b_SsMetQ, BlacR | This study |
Plasmid | ||
pET16b | Plasmid for expression of N-terminally His-tagged recombinant proteins in E. coli | Laboratory collection |
pET16b-ssMetQ | pET16b derivative encoding recombinant S. suis MetQ | This study |