Figure 5

Effects of Artemisia argyi essential oil on the microbial communities in the intestinal of recipient chicks. One-day-old chick were given 100 mg/kg/day of Artemisia argyi essential oil continuously for 7 days, and faecal microbiota was evaluated using 16S rRNA sequencing on day 7. A–D Chao1 index (A), Shannon index (B), Simpson index (C), and Observed_otus (D) showed that EOH group has reduced alpha-diversity, compared to the NC group (n = 6). E Vene analyses demonstrated different OTUs in the NC group and Artemisia argyi essential oil treatment group (EOH) (n = 6). F PCoA scores of chicks fecal based on unweighted unifrac scores (n = 6), indicated that the composition of the gut microbiota was different in the two groups. G–H Bacterial composition of the indicated groups at the phylum (G) and genus (H) levels (n = 6). I–K The relative abundance of Parabacteroides (I), Lactobacillus (J), and Butyricoccus (K) at genus levels (n = 6). L LEfSe was performed to show several bacterial taxa enriched in NC and EOH groups (log₁₀LDA score > 4). NC: Uninfected chick group, EOH: Continuous feeding of 100 mg/kg/day of Artemisia argyi essential oil for 7 days. Data are expressed as the mean ± SD. **P < 0.01 vs the NC group.