Figure 6

Effects of supplementation with L. reuteri against S. pullorum-infected chicks. A Schematic diagram of L. reuteri fed to treat S. pullorum-infected chicks. Chicks were treated with L. reuteri for 14 days, and challenged with S. pullorum on days 8–10. B Protective effect of L. reuteri feeding against bacterial colonization of the visceral of S. pullorum-infected chicks (n = 6). C Effects of L. reuteri supplementation on the morphological structure of the ileum intestine of S. pullorum-infected chicks. D–F The villus height (D), crypt length (E), and ratio of the villus to crypt length (F) of the ileum (n = 3). G–J Gene expression levels of Claudin-1 (G), MUC-2 (H), Occludin (I), and ZO-1 (J) in chick ileum were determined by qPCR, and normalised by β-actin expression (n = 3). K–P Determination of inflammatory factor IL-1β (K), IL-6 (L), IL-10 (M), NF-κB (N) and SOD (O), MDA (P) levels in chick ileum by ELISA (n = 6). Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01 vs the S. pullorum infection (SP) group.