Figure 7

Supplementation with Artemisia argyi essential oil or L. reuteri activates the Wnt/β-catenin signalling pathway in S. pullorum-infected chicks. A and E Supplementation with L. reuteri (A) or Artemisia argyi essential oil (E) upregulated mRNA expressions of genes associated with intestinal epithelial differentiation during S. pullorum infection (n = 3). B and F The comparison of Wnt/β-catenin signalling genes expression in the ileum of L. reuteri (B) or Artemisia argyi essential oil (F) treated S. pullorum-infected chicks (n = 3). C and G The expression levels of Notch signalling genes in the ileum of S. pullorum-infected chicks treated with L. reuteri (C) or Artemisia argyi essential oil (G) were measured by qPCR (n = 3). D and H L. reuteri (D) or Artemisia argyi essential oil (H) treatment increased the expression of intestinal protective genes in the ileum of S. pullorum-infected chicks (n = 3). Data are expressed as the mean ± SD. ns P > 0.05, *P < 0.05, **P < 0.01 vs the S. pullorum infection (SP) group.